RNA
Part:BBa_K3907001
Designed by: Laura Keffer-Wilkes Group: iGEM21_Lethbridge_HS (2021-10-12)
siRNA1_scramble against ADT2
This construct contains 26 repeating copies of a 19 bp fragment that is a scrambled version of BBa_K3907000 against the Arabidopsis gene ADT2. The scramble sequence can be used as a control to test the efficacy of the original siRNA construct. The DNA is to be used as a template to create siRNA fragments using RNase H and DNA chimer splints as per Feyrer et al. (2020).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |